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The nontranscribed chicken calmodulin pseudogene cross-hybridizes with mRNA from the slow-muscle troponin C gene.

机译：未转录的鸡钙调蛋白假基因与慢肌肌钙蛋白C基因的mRNA交叉杂交。

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摘要

A chicken calmodulin pseudogene with no introns was previously shown to hybridize under stringent conditions with an mRNA species present in skeletal and cardiac muscles, yet it would not hybridize to calmodulin mRNA (J. P. Stein, R. P. Munjaal, L. Lagace', E. C. Lai, B. W. O'Malley, and A. R. Means, Proc. Natl. Acad. Sci. USA 80:6485-6489, 1983). Using the pseudogene as a probe, we isolated a full-length cDNA corresponding to this mRNA from a chicken breast muscle library and showed by sequence analysis that it encodes slow-muscle troponin C and not the pseudogene product. Hybridization between the calmodulin pseudogene and slow-muscle troponin C cDNA is due to a short region of high homology in those nucleotides that encode helices B and C of troponin C and calmodulin. Genomic Southern analysis showed the calmodulin pseudogene and the gene for slow-muscle troponin C to exist as distinct single copies.

机译：先前显示无内含子的鸡钙调蛋白假基因可在严格条件下与骨骼肌和心肌中存在的mRNA物种杂交，但不会与钙调蛋白mRNA杂交（JP Stein，RP Munjaal，L.Lagace'，EC Lai，BW O'Malley和AR Means，美国国家科学院院刊80：6485-6489，1983）。使用假基因作为探针，我们从鸡胸肌文库中分离出了与该mRNA相对应的全长cDNA，并通过序列分析显示其编码慢肌钙蛋白C而不是假基因产物。钙调蛋白假基因和慢肌肌钙蛋白C cDNA之间的杂交是由于编码肌钙蛋白C和钙调蛋白的螺旋B和C的那些核苷酸中具有高度同源性的短区域引起的。 Southern基因组分析显示钙调蛋白假基因和慢肌肌钙蛋白C基因以不同的单拷贝形式存在。

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Putkey, J A; Carroll, S L; Means, A R;
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年度 1987
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1. The nontranscribed chicken calmodulin pseudogene cross-hybridizes with mRNA from the slow-muscle troponin C gene. [J] . J A Putkey, S L Carroll, A R Means Molecular and Cellular Biology . 1987,第4期

机译：非筛选的鸡钙调蛋白假蛋白与来自缓慢肌肉肌钙蛋白C基因的mRNA交叉杂交。
2. The nontranscribed chicken calmodulin pseudogene cross-hybridizes with mRNA from the slow-muscle troponin C gene. [J] . J A Putkey, S L Carroll, A R Means Molecular and Cellular Biology . 1987,第4期

机译：非筛选的鸡钙调蛋白假蛋白与来自缓慢肌肉肌钙蛋白C基因的mRNA交叉杂交。
3. The nontranscribed chicken calmodulin pseudogene cross-hybridizes with mRNA from the slow-muscle troponin C gene. [J] . J A Putkey, S L Carroll, A R Means Molecular and Cellular Biology . 1987,第4期

机译：非筛选的鸡钙调蛋白假蛋白与来自缓慢肌肉肌钙蛋白C基因的mRNA交叉杂交。
4. EFFECTS OF ENDOGENOUS PROTEASES EFFECTORS ON THE DEGRADATION OF TROPONIN-T AND DESMIN OF CHICKEN BRISKET MUSCLE DURING POSTMORTEM AGING1 [C] . Z.Gaile . 2007

机译：内脏蛋白酶效应对鸡RIS骨肌老化过程中肌钙蛋白-T和蛋白质降解的影响1
5. Studies of calcium and magnesium binding and exchange with mutants of troponin C and calmodulin and characterizing the interactions of calmodulin with TRP calcium channels [D] . Tikunova, Svetlana Borisovna 2000

机译：钙和镁与肌钙蛋白C和钙调蛋白突变体的结合和交换以及钙调蛋白与TRP钙通道相互作用的研究
6. The nontranscribed chicken calmodulin pseudogene cross-hybridizes with mRNA from the slow-muscle troponin C gene. [O] . J A Putkey, S L Carroll, A R Means 1987

机译：未转录的鸡钙调蛋白假基因与慢肌肌钙蛋白C基因的mRNA交叉杂交。
7. Tissue-specific expression of a chicken calmodulin pseudogene lacking intervening sequences. [O] . Stein, J P, Munjaal, R P, Lagace, L, 1983

机译：缺乏中介序列的鸡钙调蛋白假基因的组织特异性表达。
8. Neutron and x-ray scattering studies of the interactions between Ca2+-binding proteins and their regulatory targets: Comparisons of troponin C and calmodulin. [R] . Trewhella, J., Olah, G. A. 1993

机译：中子和X射线散射研究Ca2 +结合蛋白与其调节靶标之间的相互作用：肌钙蛋白C和钙调蛋白的比较。

The nontranscribed chicken calmodulin pseudogene cross-hybridizes with mRNA from the slow-muscle troponin C gene.

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